MLTS 1105

Course Requirements

FALL SEMESTER 2004

CLASS BEGINS Aug. 23 - CLASS ENDS Oct. 15

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Text: Immunology and Serology in Laboratory Medicine by Mary Louise Turgeon, 3RD Ed.,

ISBN: 0-323-02371-1

CLASS HOURS: 8:30 - 10:20a.m. - Monday, Wednesday -S103 and 13:00-15:00 Wed. S203

8:30 - 10:20a.m. - Friday (LAB) - S203

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COURSE EVALUATION:

A. A series of mostly objective and discussion tests covering material contained in both lecture and laboratory sessions.

B. Observations and evaluation of performance and proficiency demonstrated during laboratory

sessions.

C. Several laboratory practicals are also given.

D. Students write the National MLT exam (grade at college not affected in any way by exam).

E. Your class grades will be determined by tests, exams, affective evaluations, and lab practicals. No

make-up tests will be given. Two (2) missed tests equal two zeros. All grades are average for a

final grade. (Affective = 10% and test grades - 90%).

  1. An oral/written report on a subject chosen by the instructor will be required of each student. (Counts as a test grade).

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Grades: 90 - 100 A 60 - 74 D

80 - 89 B Below 60 F

*75 - 79 C

Grades will be determined by tests in class/lab and class reports (oral) = 90%. Affective Domain (work ethics) will be 10% of your final grade. (See attached) A written report (typed & double spaced with a minimum of 5 pages and correct spelling) will be required as well as your oral report. A minimum of four (4) references are needed for the bibliography that must be included. All grades are averaged; no grades are dropped. Students are expected to attend class and be on time. Three tardies = one absence. Tardy is defined as coming into the classroom after the last bell for that class has rung. After four absences the student may receive an > F= . Employers always ask about the attendance of a student and hospitals depend on employees being at their assigned work places on time. The pattern you set in your classes is usually the pattern you follow when you go to work.

Labs: Labs will be in Sequoya 203. One or more field trips may be scheduled therefore check with the Student Activities office; you need to sign a new travel waiver for Fall Semester 2004.

All students will be required to wear gloves, 3/4 or longer lab coats, face shields and closed-toe shoes while in the laboratory areas. Gloves/lab coats are furnished for the students. Goggles are available for purchase in the DSC bookstore. No open-toe shoes or shorts allowed in the lab.

All hazardous materials (blood, gauze, needles and contaminated articles) must be disposed of either in SHARPS containers or Biohazard marked (orange bags) on counters. No food, drink or smoking in lab areas. Eye wash stations are available at the front desk, sink and side counter sinks.

*See the attached sheet for full safety precautions and rules. Be sure to sign the safety sheet. A quiz will be given on laboratory safety after you view the OSHA film.

If you spill something please notify the instructor. My office is S113 and the telephone # is 272-4512. If you are having a problem with grades please see me before Mid-Term (Nov. 1). No test make-up unless prior arrangements have been made.

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WORK ETHICS COMPONENT: (AFFECTIVE DOMAIN)

Identification and demonstration of effective work ethics will be an integrated component of the course. The work ethics traits that will be identified and evaluated are:

1. Attendance: Attends class; arrives/leaves on time; notifies instructor in advance of planned absences; makes up assignments punctually.

2. Character: Displays loyalty, honesty, trustworthiness, dependability, reliability, initiative, self-discipline, and self-responsibility.

3. Teamwork: Respects rights of others; is a team worker, is cooperative; is assertive; displays a customer service attitude; seeks opportunities for continuous learning; displays mannerly behavior.

4. Appearance: Displays appropriate dress, grooming, hygiene, and etiquette.

5. Attitude: Demonstrates a positive attitude; appears self-confident; has realistic expectations of self.

6. Productivity: Follows safety practices; conserves materials; keeps work area neat and clean; follow directions/procedures.

7. Organizational Skills: Manifests skill in personal management, time management, prioritizing, flexibility, stress management, and dealing with change.

8. Communication: Displays appropriate nonverbal and verbal skills.

9. Cooperation: displays leadership skills; appropriately handles criticism and complaints; demonstrates problem-solving capability; maintains appropriate relationship with supervisors and peers; follows chain of command.

10. Respect: Deals appropriately with cultural/racial diversity; does not engage in harassment of any kind.

WORK ETHICS GRADING STRUCTURE:

3 = Exceeds expectations

2 = Meets expectations

1 = Needs improvement

0 = Unacceptable

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Term Paper: Double-spaced (typed / computer); Correct Spelling; Audio or Visual aid (at least one); Bibliography (4 required); Content: definition of disease/syndrome, etc, background, main discussion/lab tests/etc, conclusion(s); Presentation (DO NOT READ YOUR REPORT). This counts as one test grade.

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Students with disabilities or special needs of any type should contact Disability Support Services in the Ace Center to request reasonable accommodations for academic programs and other activities of Dalton State College. Students must personally register with Disability Support Services by providing adequate documentation to verify the disability and/or need. A minimum of five to ten business days is needed for approved accommodations to be arranged. The ACE Center is located on the lower level of Pope Student Center.

Doris Shoemaker

Seq. 113

272-4512

dshoemaker@em.daltonstate.edu

 

 

 

 

 

 

 

MLTS 1105

IMMUNOLOGY/SEROLOGY

Fall Semester 2004

Learning Outcomes/Objectives

After completing the Chapters 1 - 29 in the text, the student should be able to do the following with a minimum of 75% accuracy:

Define the term immunology.

Explain the function of the immune system.

Define the terms antigen and antibody.

Name and describe the characteristics of each of the five immunoglobulin classes.

Draw and describe a typical IgG molecular structure.

Describe the functions of granulocytes, monocytes-macrophages, and lymphocytes-plasma cells as components of the immune system.

Name and describe the signs and symptoms of abnormal neutrophil function.

Explain the process of phagocytosis.

Name and describe the function of primary and secondary lymphoid tissue.

Explain the function of T lymphocytes in immunity.

Explain the function of B lymphocytes in immunity.

Describe the mechanisms and consequences of complement activation.

Discuss the occupational transmission of hepatitis B virus (HBV) and human immuno-deficiency virus (HIV).

Describe the first line of defense, natural immunity, and adaptive immunity as body defense systems against microbial diseases.

Name the four phases of an antibody responses.

Describe the characteristics of a primary and secondary (anamnestic) response.

Describe the method of production of a monoclonal antibody.

Discuss the role of monocytes-macrophages in cellular immunity.

Describe the characteristics and role of acute-phase reactants in the inflammatory response.

Describe the evaluation of suspected lymphocytic or plasma cell defects.

Name and compare disorders with immunologic (lymphocytic or plasma cell) origin.

Briefly describe the assessment of complement levels.

Name and describe other nonspecific mediators of the immune system.

Describe the practice of universal precautions.

Explain the proper handling of hazardous material and waste management, including infectious waste, chemicals, and radioactive waste.

Name and compare the characteristics of at least five agglutination methods.

Name and briefly describe at least five precipitation methods.

Describe the electrophoresis technique.

State the clinical applications of immuno-electrophoresis.

Name at least three types of labels that can be used in immunoassay.

Describe the principle, advantages, and disadvantages of nephelometry.

Describe complement fixation.

Explain neutralization procedures.

Discuss the characteristics and clinical applications of cryoglobulins.

Describe important characteristics in the acquisition and development of infectious diseases.

Describe the etiology, epidemiology, signs and symptoms, immunologic manifestations, and diagnostic evaluation of Brucella abortus, Francisella tularenis, and Salmonella species, including S. paratyphi, and Rickettsial diseases.

Describe the principles of immunologic-serologic testing, including written procedural protocol, accuracy in testing, and blood specimen preparation.

Explain methods for enhancing agglutination.

Describe the characteristics of graded agglutination reactions.

Compare the advantages and disadvantages of five precipitation methods.

Compare immunoelecrophoresis (IEP) and immunofixation electrophoresis (IFE).

Briefly compare at least three immuno-fluorescent techniques.

Explain the principle of flow-cell cytometry and its clinical application.

Describe and compare radioimmunoassay (RIA), radioallergosorbent test (RAST), radioimmunosorbent test (RIST), enzyme immunoassay (EIA), and immunofluorescent techniques.

Describe and discuss the clinical applications of techniques in molecular biology.

Briefly describe the laboratory detection of immunologic responses.

Explain how the body develops immunity to bacterial, fungal, viral, rickettsial, mycoplasmal, and parasitic diseases.

Describe the principle of the febrile agglutinin procedure.

Discuss the immunologic manifestations and diagnostic evaluation of streptococcal infection.

Describe the etiology, epidemiology, and signs and symptoms of primary, secondary, latent, and late (tertiary) syphilis.

Describe the origin and manifestations of the condition, congenital syphilis.

Explain the immunologic manifestations and diagnostic evaluation of syphilis.

Discuss the immunologic manifestations and diagnostic evaluation of Lyme disease.

Discuss the etiology and epidemiology of acquired, latent, and congenital cyto-megalovirus (CMV) infection.

Explain the signs and symptoms of acquired and congenital CMV infections.

Describe the immunologic manifestations of CMV.

Describe the etiology, epidemiology, and signs and symptoms of infectious mononucleosis.

Explain the immunologic manifestations of infectious mononucleosis, including heterophil antibodies.

Name the various form of primary infectious hepatitis.

Describe the etiology and epidemiology of rubella infection.

Compare the immunologic manifestations of acquired and congenital rubella infection.

Describe the etiology, epidemiology, signs and symptoms, and complications of streptococcal infection.

Explain the principles of the antistreptolysin O titer (ASO) and rapid latex agglutination anti-streptolysin-O procedures.

Discuss the principles and clinical applications of the qualitative and quantitative VDRL procedure.

Discuss the principles and clinical applications of the fluorescent Treponemal pallidum antibody absorption test (FTA-ABS).

Describe the etiology, epidemiology, and signs and symptoms of Lyme disease.

Explain the treatment and prevention of Lyme disease.

Analyze case studies of Lyme disease.

Name and explain the serologic markers and diagnostic evaluation of CMV.

Discuss the principles and applications of the passive latex agglutination and quantitative determination of IgM antibody.

Discuss the elements of EBV serology and the diagnostic clinical applications of the presence of each component.

Compare the serologic procedures and clinical applications of the Paul-Bunnell, Davidson differential, and Monospot techniques.

Compare the etiology, epidemiology, signs and symptoms, laboratory evaluation, and prevention of the major forms of infectious hepatitis.

Explain the signs and symptoms of acquired and congenital infection.

Describe the etiology and epidemiology of toxoplasmosis.

Explain the signs and symptoms of acquired and congenital toxoplasmosis infection.

Describe the etiology and viral characteristics of human immunodeficiency virus (HIV-1).

Discuss the signs and symptoms of various stages, as well as the classification, of HIV infection.

Compare the general characteristics of monoclonal and polyclonal gammopathies.

Describe and compare the etiology, epidemiology, signs and symptoms, immunologic manifestations, and diagnostic evaluation of multiple myeloma and Waldenstrom= s primary macroglobulinemia.

Explain the role of T and B cells in autoimmunity and dysfunction.

Describe the etiology, including factors such as hormones and drugs, that influence the development of lupus erythematosus.

Explain the epidemiology and signs and symptoms of lupus.

Describe the etiology, epidemiology, and signs and symptoms of rheumatoid arthritis.

Discuss the immunologic manifestations and diagnostic evaluation of rheumatoid arthritis.

Explain the diagnostic evaluation of rubella, including hemagglutination inhibitation, passive latex agglutination, and semiquantitative immunoassay for IgG.

Discuss the immunologic manifestations and diagnostic evaluation of toxoplasmosis, including the quantitative determination of IgM antibodies to Toxoplasmosis gondii.

Describe the immunologic manifestations and cellular abnormalities of HIV-1 infection.

Explain the serologic markers and diagnostic evaluation of HIV.

Define hypersensitivity reactions, including anaphylactic reactions.

Compare types I, II, III, and IV hyper-sensitivity reactions.

Explain and contrast the characteristics of other monoclonal disorders.

Describe the nature of autoimmune disorders, including factors influencing their development.

Briefly name and describe organ-specific and mid-spectrum autoimmune disorders.

Describe the immunologic manifestations of lupus, including diagnostic evaluation.

Discuss the laboratory evaluation of antinuclear antibodies (ANAs).

Briefly describe Felty= s syndrome and juvenile rheumatoid arthritis.

Explain diagnostic procedures used in the identification and evaluation of rheumatoid arthritis.

Name and describe the histocompatibility antigens.

Explain the clinical applications of histocompatibility antigens and human leukocyte antigens (HLA).

List frequently used terms in transplantation.

Name various types of transplants.

Briefly discuss the role of tumor markers in the diagnosis and treatment of malignancy.

Explain the etiology, epidemiology, signs and symptoms, manifestations, diagnosis, and prevention of GVHD.

Describe the types of graft rejection.

Briefly explain the mechanism of organ or tissue rejection.

Define graft-versus-host disease (GVHD).